Lysosomes in Cancer Progression and Invasion
Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, The Netherlands
A 2.5 year post-doc position is available in our laboratory to study the role of lysosomes in cancer. Lysosomes, the main degradative organelle of the cell, are central in regulating the delicate balance between anabolism and catabolism. Lysosomal transformation is a long-known trait of cancer cells, but how this translates into cancer progression is not established. Within this project, we will investigate the molecules and pathways that regulate the lysosomal transformation in cancer cells using advanced microscopy, biochemistry and mass spectroscopy. The project will be part of the new research line dedicated to high-resolution understanding of organelle functions in cancer biology, led by Dr. Nalan Liv.
Qualifications and skills
- A PhD in the life sciences (cell biology, molecular biology, cancer biology, or a related field)
- Strong background in either organelle biology, cancer biology, biochemistry
- Keen interest in fluorescence and electron microscopy
- An ability to work independently, interpret, present and discuss experimental data
- Excellent communications skills
You are a highly-motivated ambitious scientist with strong quantitative research, organization and communication skills. A strong track record of research accomplishments in organelle biology, molecular biology and/or cancer biology is requested. The right applicant is able to work both independently and within a team, at national and international levels. You have an excellent command of the English language, both oral and written.
Utrecht is a beautiful city located in the center of the Netherlands, close to Amsterdam and its international airport. You will be based in the Cell Biology section of the Center for Molecular Medicine, a dynamic institute with international and interactive research groups with direct access to a large number of facilities. Local training opportunities include molecular cell biology, biochemistry, live-cell imaging, electron microscopy, and correlative light – electron microscopy. You will be appointed to the Klumperman lab, and the project is led by Dr. Nalan Liv.
Terms of employment
Your salary will be based on a full-time appointment of 36 hours, and the terms of employment are in accordance with the Collective Labour Agreement for University Medical Centers (CAO UMC).
The position can be filled per 1 April 2021.
Information regarding the lab and institute can be obtained at: http://www.cellbiology-utrecht.nl.
PhD project position
Uncovering the spatio-temporal regulation of endo- lysosomal lipid trafficking in control of metabolism
What you are going to do
The dysregulation of lipid metabolism is at the center of several human diseases, including cancer, fatty liver and cardiovascular disease. For this reason we are interested in understanding the molecular mechanisms that regulate lipid homeostasis in health and disease. As part of the European ITN consortium EndoConnect a 4 year PhD position is available to study the role of the endosomal system in regulating intracellular cholesterol levels. More specifically, we will study the molecular regulation of LDL-receptor trafficking and cholesterol exit from lysosomes in healthy and disease conditions. An important challenge will be to understand the role of CORVET and CHEVI complexes (Jonker et al., Nature Communications) in LDL-receptor recycling.
We will use a wide range of modern experimental approaches with a strong focus on microscopy, including live cell microscopy and correlative light – electron microscopy (CLEM). These will be combined with gene editing and biochemical strategies and mass spectroscopy methods.
As a PhD student within EndoConnect you will be part of an international team of young researchers. You will have your individual research project at your host organization, and to broaden your PhD training you will undertake internships at complementary EndoConnect partner organizations. In addition, you will follow advanced interdisciplinary courses by leaders in the field of molecular and cell biology, metabolism, physiology and biomedical research.
What we expect from you
We are looking for a broadly-trained and highly-motivated PhD candidate with an (almost completed) MSc in Medical Biology, Life Sciences, Biochemistry or a related field. Experience and/or affinity with microscopy is an advantage. The candidate should have good command of oral and written English. Excellent communication skills and the ability to work within an interdisciplinary and international research group are essential.
Successful candidates must fulfil the criteria defined by the European Commission:
- you have to be in the first 4 years of your research career and not been awarded a doctoral degree at the time of recruitment.
- At the time of recruitment, you may not have resided in the Netherlands for more than 12 months in the 3 years immediately prior to the reference date.
Where you are going to work
Utrecht is a beautiful city located in the center of the Netherlands, close to Amsterdam and its international airport. You will be based in the Cell Biology section of the Center for Molecular Medicine of the UMC Utrecht, a dynamic institute with international and interactive research groups and access to a large number of facilities. You will be enrolled in the PhD program of Biomembranes, which offers an interdisciplinary program to PhD students from different faculties. Research in the Klumperman group focuses on the role of the endo-lysosomal system in health and disease, including metabolic disorders, neurodegenerative diseases and cancer. The ultimate aim is to use this knowledge to manipulate endo-lysosomes for treatment of these diseases. The lab is internationally known as expert center for immuno-electron microscopy and correlative light electron microscopy (CLEM). Judith has been (co)-promotor on 18 successfully completed PhD theses, (co)-authored 165 peer-reviewed publications and is a regular speaker on international conferences.
More information can be found on these websites:
What we offer you
We offer an ambitious, international research environment with a positive, social atmosphere and ample opportunity for personal and scientific development. The lab has excellent facilities for microscopy, biochemistry and molecular biology and direct access to other technology platforms, such as proteomics. We have an expert team of technicians that will train you in the required lab skills. As part of the local and international communities involved in this project you will have ample opportunities for training and courses. Your salary will be based on a full-time appointment of 36 hours. Terms of employment are in accordance with the Collective Labour Agreement for University Medical Centers (CAO UMC).