CELL MICROSCOPY CORE

Equipment

Equipment for (immuno)-electron microscopy:

  • 1 FEI Tecnai 20 electron microscope
  • 1 Fei Tecnai 12 electron microscope
  • 1 Jeol 1010 electron microscope
  • 3 Leica ultra microtomes
  • 5 Leica ultra-cryomicrotomes
  • 1 Leica EM ICE high-pressure freezer
  • 1 Balzers critical point dryer
  • 1 Leica AFS 2 freeze substitution machine
  • 1 Leica AC20 automated grid stainer.

 
Technical details of the electron microscopes:

Microscope Max acc
voltage
Filament STEM Cryostage CCD camera Plate camera Special features
Jeol 1011 100 KV tungsten - + 2048 x 2048 + -
FEI Tecnai 12 120 KV LaB6 + + 2048 x 2048 + Tomography
low dose software
FEI Tecnai 20 200 KV LaB6 - + 4096 x 4096 +

 

Equipment for Light Microscopy:

  • 1 Leica Thunder with Tirf and infinity scanner
  • 1 Zeiss LSM700
  • 1 ECHO Revolve, color camera and fluorescent microscope
  • 1 Deltavision RT Core system equipped with lasers for FRAP, FLIP and FRET

 

Technical details light microscopes:

Optics

LSM 700

Deltavision

Thunder

CellInsight

Revolve

Lens 100x

 

yes, DIC

yes, Tirf

 

 

Lens 63x

yes, DIC

yes, DIC

yes, Water

 

yes

Lens 40x

yes

yes, DIC

yes, 2x Water, Oil

 

yes

Lens 20x

          yes

yes, DIC

yes

yes

yes

Lens 10x

 

 

yes

yes

yes

Lens 4x

 

 

 

 

yes

 

Light sources

LSM 700

Deltavision

Thunder

CellInsight

Revolve

Laserlines

405, 488, 555, 639

410, 488, 532

488, 561

 

 

Krypton lamp

 

 

 

 

 

Mercury lamp

yes

 

 

 

 

Halogen lamp

yes

 

yes

yes

yes

Xenen lamp

 

yes

     

LED lamp

 

yes

405, 488, 568, 633

405, 488, 555, 639

405, 488, 555, 639

 

Detectors

LSM 700

Deltavision

Thunder

CellInsight

Revolve

Detector

2 PMT

Coolsnap HQ + Photometrics EMCCD

Prime 95B sCMOS

CMOS

CMOS + Color camera

Max resolution

2048 x 2048

1392 x 1024 + 1024 x 1024

1608x1608

   

Frames/sec

5

max 30 +

60

   

 

Software / techniques

LSM 700

Deltavision

Thunder

CellInsight

Revolve

Software

Zen2010

Softworx

LasX

Cellomics

Echo

 

   

(Metamorph)

   

FRAP

no

yes

yes

no

no

FLIP

no

no

yes

no

no

FRET

no

yes

yes

no

no

time series

yes

yes

yes

no

no

3D

yes

yes

yes

yes

yes

Deconvolution

no

yes

yes

no

no

Computaional clearing

no

no

yes

no

no

Panels

yes

yes

yes

yes

yes

Point visiting

yes

yes

yes

yes

no

 

Additional features

LSM 700

Deltavision

Thunder

CellInsight

Revolve

Temp control

no

RT- 40°C

RT- 40°C

no

no

CO2 control

no

yes

yes

no

no

Autom. Z scan

z stepper

DV Stage

yes

yes

no

Motorized stage

yes

yes

yes

yes

no

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