The iPSC (induced pluripotent stem cell) facility is located in the stem cell (ML2) cell culture facility of the Department of Cell Biology. The facility uses a lentiviral polycistronic, excisable vector and non-integrating episomal plasmid vectors to reprogram somatic cells to iPSC. The standard cell type for reprogramming is skin fibroblasts. However, we also have successfully reprogrammed human haematopoietic stem/progenitor cells (CD34pos) and cells derived from nasal swaps. The facility has already generated patient specific iPSC-lines from several blood related disorders and chromosomal rearrangement disorders. The facility has a dedicated laminar flow cabinet and incubator. Derived iPSC are karyotyped, analysed for pluripotency markers and in vitro differentiated to show their pluripotency.
The facility also invests in optimizing reprogramming protocols (integration free, higher efficiency) and iPSC culture conditions (feeder free in chemical defined medium).
The iPSC facility is open to collaborate with researchers with good research plans that see possibilities for iPSC in their research (e.g. patient specific iPSC).