Basic course resin embedding and ultrathin sectioning
Date: January 16-18, 2017
The 3 day workshop organized in collaboration with Leica is meant for people that do not have experience with standard preparation techniques for transmission electron microscopy. This workshop is primarily practical in nature, although some topics will be dealt with in lectures. The entire procedure from (live) specimen till stained ultrathin resin section will be covered from start to finish. If you participate most of the time will be spent in the lab working with standard specimens like cultured cells or tissues. Besides embedding in a resin and managing the ultra-microtome, the preparation of tools and other necessities will be covered. We provide an excellent learning environment. When manual skills like ultrathin sectioning of resin blocks are being trained the participants will have their own ultra-microtome (often equipped with a camera) to practice on. The number of participants is limited to 10 people. We will provide you with a practical manual covering the topics of the course. The preliminary program and a more elaborate list of topics that are addressed in this course can be found here.
Available equipment: 2 x Reichert Ultracut E, 3x Leica Ultracut S, 2x Leica Ultracut T, 1x Leica UC6/FC6 ,1 x Leica UC6, 1x Leica UC7. Leica KMR2/3, Leica AC20, Leica EM AMW, Leica EM TRIM2, Leica EM ACE600, Edwards Auto 306 carbon evaporator, JEOL electron microscope, 2 FEI electron microscopes
Cell Microscopy Core: Suzanne van Dijk, Tineke Veenendaal, Despina Xanthakis, Corlinda ten Brink, Cilia de Heus, George Posthuma.
Leica: Jan de Weert
Price: € 750,- excluding hotel and dinners, including lunches.
Participants are accepted on a “first-come, first-served” basis therefore we recommend that you contact us at your earliest convenience.