Advanced course Correlative Light Electron Microscopy
Date: June 22 - 27, 2017
In the workshop we offer a balanced “mix” of lectures and practical sessions. The entire workflow from specimen to fluorescent image to immune-labeled ultrathin section will be dealt with. Under guidance of the skilled staff of the Utrecht Cell Microscopy Core Life cell CLEM and Section CLEM including the complete Tokuyasu technique will be practiced and explained to the finest detail. Biological samples and immuno-reagents will be available. The program is meant for people that already have experience in cryosectioning and immunolabeling and want to extend their expertise into CLEM. We provide an excellent learning environment. When manual skills like ultrathin cryo-sectioning are being trained the participants will have their own ultra-cryo microtome (often equipped with a camera) to practice on. The number of participants is limited to 10 people. We will provide you with a practical manual covering the topics of the course.
A more elaborate list of topics that are addressed in this course can be found here.
Available equipment: 2 x Reichert Ultracut E, 3x Leica Ultracut S/ with cryo-attachment, 2x Leica Ultracut T with cryo-attachment, 1x Leica UC6/FC6, 1 x Leica FUC/FC7, Leica AFS. Leica IGL. JEOL electron microscopes, 2 FEI electron microscopes equipped for tomography and supporting equipment.
Teachers & lecturers:
Cell Microscopy Core: Cilia de Heus, Corlinda ten Brink, Suzanne van Dijk, Tineke Veenendaal, Despina Xanthakis, George Posthuma.
Diatome: Helmut Gnaegi
The course fee of € 1500 includes all immuno-reagents, disposables etc. and lunches. Hotel is not included. The course language is English.
Participants are accepted on a “first-come, first-served” basis therefore we recommend that you contact us at your earliest convenience.
The maximum number of participants is 10.
Cell Microscopy Core, Dept. Cell Biology
University Medical Center Utrecht
Heidelberglaan 100, Rm H02.313
NL - 3584 CX Utrecht
Phone:+31-88.755.65.51 Fax: +31-30-254.17.97